MATERIALS FOR ACCELERATED PROTEIN STRUCTURE DETERMINATION
The long-term objective of this work is the development of novel materials and methods for rapid structural analysis of proteins at high resolution to enable structure-based drug design for important unmet disease targets. Our strategies utilize high affinity interactions to immobilize protein targets onto TEM grids for single particle reconstruction analysis.
SELECTED PUBLICATIONS
“Selective Capture of Histidine-tagged Proteins from Cell Lysates Using TEM Grids Modified with NTA-Graphene Oxide”, Scientific Reports 2016 6, 32500.
“Non-fouling NTA-PEG-based TEM Grid Coatings for Selective Capture of Histidine-tagged Protein Targets from Cell Lysates”, Langmuir 2016 32, 551-559.
“Single-step Antibody-based Affinity Cryo-Electron Microscopy for Imaging and Structural Analysis of Macromolecular Assemblies”, Journal of Structural Biology 2014 187, 1-9.
